El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

Tema 9. Cultivo de protoplastos e hibridación somática

Your fellow students know exactly where the pitfalls lie and what the key elements will be to your protoplastow in that module. Final concentration was protoplasts per millilitre. Thanks to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams.

Production of somatic hybrids by electrofusion in Solanum.

Cultivo de protoplastos de yuca y Stylosanthes

Genetic Resourses Conservation and Characterization Division. The effect of initial culture media. Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape.


Irregular meiosis in a cultivp hybrid between S. Plant material and protoplast isolation. RAPD and chromosomal analysis of the hybrids are also presented. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig.


Aggregations of more than two protoplasts were also observed. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Earn while you study. The other parent, the wild species S.

An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. Protoplasts fusion from S. Somatoclonal variation in potato: Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement.

New York, NY Chromosome analysis The numbers of chromosome of S. Origins of cultivated potatoes and species relationships.

Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L. Shoot regeneration started twelve weeks after calli were transferred to MS medium.


Cultivo y fusion de protoplastos [1990]

The wild species S. The same primer amplified four specific bands of, and bp in S. Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.

This technique is a tool for somatic hybrid characterization Baird et al. After that, protoplasts settled down. The fultivo study guides. Many wild Solanum species are regarded as important sources for disease resistance and tolerance cjltivo many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

Improvements in regeneration from protoplasts of potato and studies on chromosome stability. Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents.

Overspecific notes are at your disposal.